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Millipore Anti-Nestin Antibody, Clone 10c2, Alexa Fluor 488 Conjugate Detects Level Of Nestin & Has Been Published & Validated For Use In Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Product Information
Format
AlexaFluor®488
Control
RNcell CX neural stem cells (SCC007).
Presentation
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Quality Level
MQ100
Applications
Application
Anti-Nestin Antibody, clone 10C2, Alexa Fluor 488 conjugate detects level of Nestin & has been published & validated for use in ICC.
Key Applications
Immunocytochemistry
Biological Information
Immunogen
Fusion protein corresponding to human Nestin.
Clone
10C2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.
Isotype
IgG1
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_006608
Gene Symbol
NES
Nbla00170
FLJ21841
Nestin
Purification Method
Protein A Purfied
UniProt Number
P48681
Molecular Weight
The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDA.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
