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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M | ELISA, ICC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5328 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-RAGE Antibody, clone DD/A11 or A11 |
| Alternate Names |
|
| Background Information | RAGE (Receptor for Advanced Glycosylation End Products) is a 35 kDa cell surface receptor that binds molecules modified by advanced glycation end products (AGEs), an irreversible non-enzymatic reaction occuring from the interaction of proteins and lipids with glucose. The increased expression of RAGE is associated with many disorders, such as diabetic vasculopathy, neuropathy, retinopathy and neuropathy, Alzheimer’s disease and immune/inflammatory reactions of the vessel walls. AGE/RAGE accumulates at sites of vascular disease in diabetes, but blockade of RAGE suppresses development of atherosclerosis and vascular disorders. RAGE binds to amyloid beta, which is overproduced in neurons and vessels in Alzheimers disease, and this interaction results in oxidative stress leading to neuronal degeneration. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2a in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-RAGE Antibody, clone DD/A11 or A11 is an antibody against RAGE for use in ELISA, IC, IH & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot of this antibody was shown to be reactive on paraffin embedded tissue sections. Immunocytochemistry: A previous lot was shown to work on cells fixed with ice cold acetone using a Cy3 conjugated secondary antibody. ELISA: A previous lot was shown to work on direct ELISA. Immunoblotting: Recognizes a band of ~48kDa in bovine lung extract. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant truncated extracellular part of RAGE produced in E. coli. |
| Epitope | Extracellular |
| Clone | DD/A11 or mAbA11 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | RAGE (Receptor for Advanced Glycosylated End Products). The antibody recognizes both natural and recombinant RAGE. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Expected to react with rat based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q15109 # Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Receptor for amyloid beta peptide. SIZE: 404 amino acids; 42803 Da SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. TISSUE SPECIFICITY: Endothelial cells. PTM: Autophosphorylated (By similarity). SIMILARITY: SwissProt: Q15109 # Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| Molecular Weight | 48 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot: 1:500 dilution of this lot detected RAGE on 10 μg of Mouse Brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |