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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Mk | WB, ICC, IHC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5416 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Glutamate Receptor 3 Antibody, clone 3B3 |
| Alternate Names |
|
| Background Information | AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition.There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. The insertion or removal of GluR1/GluR4 oligomeric channels from postsynaptic membranes appears to be LTP/LTD activity dependent while GluR2/GluR3 oligomers are continuously cycling. Millipore’s anti-GluR2/3 antibody is useful for localizing expression of GluR2/GluR3 receptor assemblies in the PSD. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquid in PBS. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Glutamate Receptor 3 Antibody, clone 3B3 is an antibody against Glutamate Receptor 3 for use in WB, IC, IH, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot of this antibody was used in IH. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Fusion protein, N-terminus of GluR3 |
| Epitope | N-terminus |
| Clone | clone 3B3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with Glutamate Receptor 3 (GluR3). No cross reactivity with GluR2. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human, mouse and monkey. Expected to react with rat based on sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->g). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt:P42263 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. SIZE: 894 amino acids; 101,157 Da SUBUNIT:Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with PRKCABP, GRIP1 and GRIP2. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-621 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-847 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. DISEASE INVOLVEMENT: Defects in GRIA3 are the cause of mental retardation X-linked type 94 (MRX94) [MIM:300699]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRX94 patients have moderate mental retardation. Other variable features are macrocephaly, seizures, myoclonic jerks, autistic behavior, asthenic body habitus, distal muscle weakness and hyporeflexia. SIMILARITY: SwissProt: P42263 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family. |
| Molecular Weight | ~ 110 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Western Blot Analysis: A previous lot of this antibody was used in WB. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |