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Millipore This Anti-Cytochrome P450 Aldosterone Synthase Antibody, Clone 1g5 Is Validated For Use In Elisa, Ic, Ih(P), Wb For The Detection Of Cytochrome P450 Aldosterone Synthase. - Mill (Additional S&H or Hazmat Fees May Apply)
Aldosterone synthase (or 18-hydroxylase) is a steroid hydroxylase cytochrome P450 oxidase enzyme involved in the generation of aldosterone. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. (Pascoe, L, et al. (1992) "Mutations in the human CYP11B2 (aldosterone synthase) gene causing corticosterone methyloxidase II deficiency." PNAS, USA. 89:496-5000).
Product Information
Format
Culture Supernatant
Presentation
Unpurified tissue culture supernatant containing 50% glycerol and 0.1% sodium azide as a preservative.
Quality Level
MQ100
Applications
Application
This Anti-Cytochrome P450 Aldosterone Synthase Antibody, clone 1G5 is validated for use in ELISA, IC, IH(P), WB for the detection of Cytochrome P450 Aldosterone Synthase.
Key Applications
ELISA
Immunocytochemistry
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Western blot: recognizes ~48kDa band in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable. Immunohistochemistry: 1:50-1:200 , ABC, enzymatic detection; clone works best with STF fixed tissues that are paraffin embedded. STF is available from Streck Laboratories, www.streck.com. Formalin fixation has been successfully reported but working dilutions are much lower (see Fujisawa, et al). Recommended counter stain is Gills Haematoxylin. Immunocytochemistry: acetone fixed cells, 1:20-1:200. ELISA Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Aldo-1-MAP (aa 175-192) (KVRQNARGSLTMDVQQSL-MAP)
Clone
1G5
Host
Mouse
Specificity
Rat cytochrome p450 aldosterone synthase
Isotype
IgG1κ
Species Reactivity
Mouse
Rat
Antibody Type
Monoclonal Antibody
UniProt Number
P30099
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
