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Millipore This Anti-Cytomegalovirus Antibody, Blend, Clone 8b1.2, 1g5.2 & 2d4.2 Is Validated For Use In If, Ih For The Detection Of Cytomegalovirus. - Mill (Additional S&H or Hazmat Fees May Apply)
Anti-Cytomegalovirus Antibody, blend, clone 8B1.2, 1G5.2, and 2D4.2
Alternate Names
CMV
Product Information
Format
Purified
Presentation
0.02 M PB, 0.25M NaCl, PH 7.6 with 0.1% Sodium Azide.
Quality Level
MQ300
Applications
Application
This Anti-Cytomegalovirus Antibody, blend, clone 8B1.2, 1G5.2 & 2D4.2 is validated for use in IF, IH for the detection of Cytomegalovirus.
Key Applications
Immunofluorescence
Immunohistochemistry
Application Notes
Immunohistochemistry
Immunofluorescence
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
MRC-5 infected cells with ATCC VR538 and AD169, ultrasonicated, screened by plate reduction neutralization tests.
Epitope
blend
Clone
8B1.2, 1G5.2, and 2D4.2
Host
Mouse
Specificity
Reacts with Immediate early, early, and late antigen preparations. Can detect CMV infection within 2 hours post-infection exhibiting a nuclear staining which reaches peak intensity between 48 and 96 hours. This antigen is persisting and can be detected during the complete CMV infection cycle. Staining of CMV structural antigens becomes apparent at about 48 hours. Extra-nuclear staining is first seen in the region of the golgi apparatus followed subsequently by staining of viral proteins and particles in the cytoplasm.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at +2-8C in convenient aliquots for up to 6 months. Do not freeze.
