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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IF, WB, ICC, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB8131 |
| Replaces | MAB8130 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Supplied in PBS buffer, pH 7.4. Contains 0.1% sodium azide and carrier protein. Has been sterile filtered. |
| Quality Level | MQ300 |
| Applications | |
|---|---|
| Application | Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2 is an antibody against Cytomegalovirus for use in IF, WB, IC, IH(P). |
| Key Applications |
|
| Application Notes | Immunohistochemistry. Immunocytochemistry. Western Blot 1:100 IFA at 1:600-1:1,200 on acetone fixed cells. Works on paraffin embedded tissue sections. Final working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Affinity purified early antigen from MRC-5 cells infected with CMV AD169 (ATCC). |
| Epitope | immediate early |
| Clone | 6F8.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with an immediate early protein M.W. 68-72 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and/or intranuclear inclusion staining which reaches peak intensity between 8-24 hours. With CMV the antigens expressed at different times are listed as: Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD. Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis. Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at +4°C for up to 12 months |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |