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Millipore This Anti-Bacterial Peptidoglycan Antibody, Clone 3f6b3 Is Validated For Use In Db, ELISA, Ic For The Detection Of Bacterial Peptidoglycan. - Mill (Additional S&H or Hazmat Fees May Apply)
This Anti-Bacterial Peptidoglycan Antibody, clone 3F6B3 is validated for use in DB, ELISA, IC for the detection of Bacterial Peptidoglycan.
Key Applications
DOT Blot
ELISA
Immunocytochemistry
Application Notes
May be used in solid-phase or competitive ELISA, immunodot-blot and immunocytochemistry formats. Although PG is a bacterial surface component, pre-treatments, such as treatment with strong acid (25mM HCl), for Gram-positive bacteria, or with a detergent, such as SDS or Triton-X (1% in PBS), for Gram-negative bacteria, may be necessary to expose the epitopes.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Insoluble PG obtained by TCA-heat and ethanol extraction of Streptococcus mutans BHT cells.
Clone
3F6B3
Host
Mouse
Specificity
Specific for the three-dimensional polymer complex structure of PG. The epitopes appear to consist of discontinuous glycan and/or amino acid residues, and have not been fully defined. In a competitive immunoassay format, several compounds were found to be ineffective as inhibitors: muramyldipeptide, N-acetylglucosamine (NAG), chitin (polymer of NAG) and acid-hydrolyzed chitin (NAG oligomers).
Isotype
IgG1
Species Reactivity
Bacteria
Antibody Type
Monoclonal Antibody
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
