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Millipore Mouse Igg1 Negative Control Monoclonal Antibody Validated For Use In Flow Cytometry & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Information
Format
Purified
Presentation
Protein A Purified mouse immunoglobulin in phosphate buffered saline, pH 7.4, containing 0.2% bovine Serum albumin and 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Key Applications
Flow Cytometry
Immunofluorescence
Application Notes
Mouse IgG1 is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.
SUGGESTED USAGE
The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.
Biological Information
Immunogen
Chicken immunoglobulin
Clone
Ci4
Concentration
0.5 mg/mL
Host
Mouse
Specificity
Reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.
Isotype
IgG1
Antibody Type
Monoclonal Antibody
Purification Method
Protein A Purfied
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
