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Millipore Mouse Igg1 Negative Control Monoclonal Antibody Validated For Use In Flow Cytometry & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Purified immunoglobulin. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Quality Level
MQ100
Applications
Application
Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Key Applications
Flow Cytometry
Immunofluorescence
Application Notes
Fluorescein conjugated mouse IgG1 is a monoclonal antibody intended for use as a negative control in immunofluorescence and flow cytometry. Its use enables an estimation of non-specific binding of fluorescein conjugated mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.
SUGGESTED USAGE
Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
Biological Information
Immunogen
Chicken immunoglobulin
Clone
Ci4
Host
Mouse
Specificity
This antibody reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.
Isotype
IgG1
Antibody Type
Monoclonal Antibody
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
