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Millipore Mouse Igm Negative Control Monoclonal Antibody Validated For Use In Flow Cytometry & Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Purified from mouse ascitic fluid. The antibody is supplied in 0.5ml 25mM Tris HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.
Quality Level
MQ100
Applications
Application
Mouse IgM Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Key Applications
Flow Cytometry
Immunofluorescence
Application Notes
The use of this antibody enables an estimation of non-specific binding of mouse monoclonal antibodies of isotype IgM to cell surface components in human blood and bone marrow. It is not suitable for use in immunohistochemical procedures.
SUGGESTED USAGE
The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used. Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
Biological Information
Immunogen
Protein I preparation of Neisseria gonorrhoeae.
Clone
GC323
Concentration
0.5 mg/mL
Host
Mouse
Specificity
This antibody reacts specifically with the outer membrane protein I component of various strains of Neisseria gonorrhoeae and does not react with any human cell surface component.
Isotype
IgM
Antibody Type
Monoclonal Antibody
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.