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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1021 |
| Description | Anti-g-CSF-R/CD114 Antibody, clone 5A11.1 |
| Alternate Names |
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| Background Information | Granulocyte colony-stimulating factor receptor (UniProt Q99062; also known as CD114, g-CSF-R, g-CSF receptor) is encoded by the CSF3R (also known as GCSFR) gene (Gene ID 1441) in human. CD114/g-CSF-R belongs to the type I subgroup of the superfamily of cytokine receptors. g-CSF-R mediates granulocyte proliferation and differentiation in both normal and malignant hematopoiesis. g-CSF-R functions as the receptor for granulocyte colony-stimulating factor (g-CSF) and is activated via ligand-induced homodimerization. The g-CSF/GCSF-R complex mediates survival, proliferation, and maturation of neutrophils and regulates granulopoiesis under both normal and stress conditions. Cell signaling via g-CSF-R is transduced primarily via the Jak/STAT pathway, but the Ras/Raf/MAP kinase and PKB/Akt pathways are also involved. CSF3R gene mutations are well documented in congenital neutropenia as well as the associated predisposition to myelodysplasia and acute myeloid leukemia myeloproliferative disorders. Human g-CSF-R is produced with a signal peptide sequence (a.a. 1-24), the removal of which yields the mature single transmembrane (a.a. 628-650) receptor with a C-terminal cytoplasmic tail (a.a. 651-836) and a large N-terminal extracellular sequence (a.a. 25-627) composed of a C2-type Ig-like domain (a.a. 25-117) and five type-III fibronectin domains (a.a. 125-623). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-g-CSF-R Antibody, clone 5A11.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of CD114. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected g-CSF-R/CD114 in human placenta and bone marrow tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human g-CSF-R/CD114 cytoplasmic domain fragment. |
| Epitope | Cytoplasmic domain. |
| Clone | 5A11.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5A11.1 was raised against a cytoplasmic domain fragment of the canonical isoform GCSFR-1. Cross-reactivity of this monoclonal antibody toward spliced isoforms GCSFR-2, -3, and -4 is likely, but has not been confirmed. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | ~43.2 kDa observed for fragment. 89.62 kDa (isoform GCSFR-1), 82.58 kDa (isoform GCSFR-2), 92.58 kDa (isoform GCSFR-3), 84.07 kDa (isoform GCSFR-4; D7) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of GST-tagged human g-CSF-R cytoplasmic domain fragment. Western Blotting Analysis: 1.0 µg/mL of this antibody detected 50 ng of GST-tagged recombinant human g-CSF-R cytoplasmic domain fragment. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |