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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1037 |
| Description | Anti-Sp100 Antibody, clone 2H1.1 |
| Alternate Names |
|
| Background Information | Nuclear autoantigen Sp-100 (UniProt P23497; also known as Nuclear DOT-associated Sp100 protein, Speckled 100 kDa) is encoded by the SP100 gene (Gene ID 6672) in human. The SP100 family genes, including SP100, SP110, SP140 and SP140L, encode highly similar proteins mainly expressed in leukocytes. Interferons (IFNs) and other viral infection-related stimuli induce SP100 family gene expression and mutations of SP100 genes are linked to immune diseases and cancers. SP100 colocalizes with promyelocytic leukemia (PML) protein in nuclear bodies (NBs), also known as nuclear domain 10 (ND10), that function to regulate gene transcription via chromatin-based mechanisms and thereby mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and defense against viral infections. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Sp100 Antibody, clone 2H1.1 is an antibody against Sp100 for use in Western Blotting, Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Sp100 nuclear foci in HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human Sp100 internal fragment. |
| Epitope | Internal (N-terminal half). |
| Clone | 2H1.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2H1.1 targeted epitope region is present in all seven human Sp100 spliced isoforms reported by UniProt (P23497). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | ~100/78 kDa observed. 100.3 kDa (isoform Sp100-HMG/SP100HMG/SpAlt-HMG), 53.64 kDa (isoform Sp100-A/SP100A/SP100), 78.43 kDa (isoform Sp100-B/SP100B/SpAlt-212), 101.4 kDa (isoform Sp100-C/SP100C), 52.98 kDa (isoform SpAlt-C), 50.46 kDa (isoform 6), and 49.90 kDa (isoform 7) calculated (with Met1 removed). Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 nuclear extract. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Sp100 in 10 µg of HepG2 nuclear extract. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |