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Millipore Anti-Ephrin-B2, Clone 10h1.1, Cat. No. Mabc127, Is A Mouse Monoclonal Antibody That Detcets Ephrin-B2 And Has Been Tested For Use In Immunohitochemistry And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Ephrin-B2 is also called EPH-related receptor tyrosine kinase ligand 5 (LERK-5) and HTK ligand (HTK-L). Ephrin-B2 is a cell surface transmembrane ligand for the Eph tyrosine kinase family of receptors. Ephrin-B2 binds Eph receptors on adjacent cells, leading to contact-dependent bidirectional signaling, which is critical for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Ephrin-B2 is expressed in lung and kidney and together with EPHB4 is involved in heart morphogenesis and angiogenesis.
Product Information
Format
Purified
Control
Mouse lung tissue lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Ephrin-B2, clone 10H1.1, Cat. No. MABC127, is a mouse monoclonal antibody that detcets Ephrin-B2 and has been tested for use in immunohitochemistry and Western Blotting.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Ephrin-B2 in rat and human lung tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Ephrin-B2 in IHC.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human Ephrin-B2.
Clone
10H1.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgMκ
Species Reactivity
Human
Mouse
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_004084
Gene Symbol
EFNB2
EPLG5
HTKL
LERK5
UniProt Number
P52799
Molecular Weight
~65 kDa observed. The calculated molecular weight of this protein is ~37 kDa; however, this protein has been observed at 65-70 kDa (Attwood, B. K., et al. (2011). Nature. 473(7347):372–375.; Lin, K. T., et al. (2008). J Biol Chem. 283(43):28969-28979.).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse lung tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ephrin-B2 in 10 µg of mouse lung tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
