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Millipore This Anti-Nrf-1 Antibody, Clone 10b11.1 Is Validated For Use In Western Blotting For The Detection Of Nrf-1. - Mill (Additional S&H or Hazmat Fees May Apply)
Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-NRF-1 Antibody, clone 10B11.1 is validated for use in Western Blotting for the detection of NRF-1.
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged recombinant human NRF-1 internal fragment.
Epitope
Internal (N-terminal half).
Clone
10B11.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Immunogen construct region is present in human NRF-1 spliced isoform Long, isfoform Short, and isfoform 4, but is more than 40% absent from spliced isoform 4 reported by UniProt (Q16656).
Isotype
IgG2bκ
Species Reactivity
Mouse
Species Reactivity Note
Mouse. Predicted to react with Human based on 100% sequence homology.
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected NRF-1 in 10 µg of NIH/3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
