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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC182 |
| Description | Anti-NRF-1 Antibody, clone 10B11.1 |
| Alternate Names |
|
| Background Information | Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-NRF-1 Antibody, clone 10B11.1 is validated for use in Western Blotting for the detection of NRF-1. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human NRF-1 internal fragment. |
| Epitope | Internal (N-terminal half). |
| Clone | 10B11.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Immunogen construct region is present in human NRF-1 spliced isoform Long, isfoform Short, and isfoform 4, but is more than 40% absent from spliced isoform 4 reported by UniProt (Q16656). |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein g purified. |
| UniProt Number |
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| Molecular Weight | ~58 kDa observed. 53.54/53.57 kDa (human/mouse isoform Long), 46.16 kDa (human/mouse isoform Short), 36.05 kDa (human isoform 3), 55.67 kDa (human isoform 4) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in NIH/3T3 cell lysate. Western Blotting Analysis: 0.25 µg/mL of this antibody detected NRF-1 in 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |