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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC200 |
| Description | Anti-APC Antibody, clone CC-1 |
| Alternate Names |
|
| Background Information | Adenomatous polyposis coli protein (APC) is a ubiquitous multidomain protein that has a well documented role as a tumor suppressor. The mechanism of APC-mediated tumor suppression is still an area of active investigation; however, several studies suggest that APC is a negative regulator of the Wnt signaling pathway as it downregulates β-catenin via interactions with Axin/GSK3-β complex, thereby preventing transcription of genes such as MYC that contribute to cell proliferation. Defective APC proteins contribute to the initiation and proliferation of various types of cancers, including familial adenomatous polyposis. However, other studies have shown that APC interacts with a range of other proteins such as the EB1 microtubule-binding proteins, to regulate other processes such as cell migration. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-APC Antibody, clone CC-1 detects levels of APC proteins & has been published & validated for use in IHC. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APC in human brain tissue containing multiple schlerosis lesions (Saikali, P. et Al. (2007). J Neurosci. 27(5):1220-1228.). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected APC in rat spinal cord injury tissue (McTique, D. M., et Al. (2001). J Neurosci. 21(10):3392-3400.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human APC. |
| Clone | CC-1 |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | 311 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected APC in human colorectal cancer tissue, human smooth muscle tissue, and human colon tissue. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |