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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB, IF, ACT, Inhibition | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC291 |
| Replaces | MABN1035 |
| Description | Anti-PSMA Antibody, clone 3/A12 |
| Alternate Names |
|
| Background Information | Prostate-specific membrane antigen (PSMA), also known as Glutamate carboxypeptidase II (GCPII), Folate hydrolase 1 (FOLH1), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I), is a member of the peptidase M28 family and is involved in prostate tumor progression. It is highly expressed in prostate epithelium and detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, and brain (at protein level). Mutations in PSMA have been associated with prostate cancer and hyperhomocysteinemia. PSMA may be a possible marker for neurological disorders such as schizophrenia, Alzheimers disease, and Huntington disease. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PSMA Antibody, clone 3/A12 is a highly specific mouse monoclonal antibody, that targets PSMA & has been tested in Flow Cytometry, western blotting, Immunofluorescence, Activity Assay & Inhibition. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot from an independent laboratory detected purified PSMA in WB (Elsasser-Beile, U., et Al. (2006). Prostate. 66(13):1359-1370.). Immunofluorescence Analysis: A representative lot from an independent laboratory detected PSMA in LNCaP cells (Elsasser-Beile, U., et Al. (2006). Prostate. 66(13):1359-1370.). Inhibition Assay Analysis (Small-animal PET imaging): A representative lot from an independent laboratory blocked PSMA in mice bearing PSMA-positive C4-2 tumors and negative DU145 tumors (Elsasser-Beile, U., et Al. (2009). J Nucl Med. 50(4):606-611.). Activity Assay Analysis: A representative lot from an independent laboratory was co-internalized with cell adherent PSMA, when PSMA binds to its putative ligand (Liu, J. et Al. (2009). Mol Pharm. 6(3):959-970.). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human PSMA. |
| Clone | 3/A12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | 84 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in 1X10E6 LNCaP cells. Flow Cytometry Analysis: 1 µg of this antibody detected PSMA in LNCaP cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |