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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IP, ICC, IHC, FC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC39 |
| Description | Anti-LAMP-1 (CD107a) Antibody, clone 1D4B |
| Alternate Names |
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| Background Information | Lysosome-associated membrane protein 1 (LAMP-1) is a highly glycosolated, single-pass type 1 membrane protein belonging to the lysosome- associated membrane protein family; a group of proteins that make up the majority of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-1 functions to traffic carbohydrate ligands to selectins. It is also thought to play a role in tumor cell metastasis. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect LAMP-1 (CD107a) using this Anti-LAMP-1 (CD107a) Antibody, clone 1D4B validated for use in WB, IP, IC, IH, FC. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Chen, 1985). Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Chen, 1985). |
| Biological Information | |
|---|---|
| Immunogen | NIH/3T3 plasma membrane fraction of mouse LAMP-1. |
| Clone | 1D4B |
| Host | Rat |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g |
| UniProt Number |
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| UniProt Summary | FUNCTION: Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane. PTM: O- and N-glycosylated; some of the N-glycans attached to LAMP-1 are polylactosaminoglycans (By similarity). SEQUENCE SIMILARITIES: Belongs to the LAMP family. |
| Molecular Weight | ~ 100 kDa observed. The calculated molecular weight of this protein is 44 kDa. The protein is heavily glycosolated and therefore its molecular weight is typically noted from 95-115 kDa. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 1:50,000 dilution of this antibody detected LAMP-1 in 10 µg of NIH/3T3 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |