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Millipore This Anti-Atg13 Antibody, Clone 2h4.2 Is Validated For Use In Western Blotting, Ihc For The Detection Of Atg13. - Mill (Additional S&H or Hazmat Fees May Apply)
The autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.
Product Information
Format
Purified
Control
Campthothecin treated HeLa cell lysate.
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blot Analysis: A representative lot detected Atg13 in HeLa cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Atg13.
Clone
2H4.2
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001136145
Gene Symbol
ATG13
Purification Method
Protein g Purified
UniProt Number
O75143
Molecular Weight
~60 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in campthothecin treated HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 µg of campthothecin treated HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
