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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, IF | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC547 |
| Replaces | MAB3192 |
| Description | Anti-Poly ADP-ribose Antibody, clone 10H |
| Background Information | Poly(ADP-ribose) polymerase is an abundant nuclear enzyme that catalyses the synthesis of poly(ADP-ribose) from nicotinamide adenine dinucleotide (NAD+). Poly(ADP-ribose) has an N-terminal DNA-binding domain containing two zinc-fingers, which is linked to the C-terminal NAD+-binding domain by a short region containing several glutamic acid residues that are sites of auto-poly (ADP-ribosyl) ation. Production of poly(ADP-ribose) within the cell is initiated by agents that generate DNA strand interruptions. The branched homopolymer chains may REACH a length of 200–300 residues but are rapidly degraded after synthesis. The function of poly(ADP-ribose) synthesis is not clear, although it seems to be required for DNA repair. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect PARP using this mouse monoclonal antibody, Anti-Poly ADP-ribose Antibody, clone 10H validated for use in western blotting, IP & Immunofluorescence. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to immunoprecipitate Poly ADP-ribose in cultured supernatents from mouse erythrocytes coated with Poly ADP-ribose (Kawamitsu, H., et Al. (1984). American Chemical Society. 3771-3777). Immunofluorescence Analysis: A representative lot was used by an an independent laboratory to detect Poly (ADP-ribose) synthesis on transfected CV-1 cells via indirect immunofluorescence (J.H. Kupper, et Al. J. Biol. Chem. (1990) 265:18721). |
| Biological Information | |
|---|---|
| Immunogen | Corresponding to human Poly ADP-ribose chain. |
| Clone | 10H |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG3κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein g Purified |
| Molecular Weight | Observed molecular weight is the result of poly(ADP-ribose) polymer on protein receptors (such as histones and transcription factors). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in untreated and UV treated HeLa cells. Western Blotting Analysis: 1.0 µg/mL of this antibody detected UV treated HeLa cells. Little or no signal observed in untreated HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |