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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC612 |
| Description | Anti-Procadherin FAT1 Antibody, clone C257 |
| Alternate Names |
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| Background Information | Procadherin FAT1 protein is a cell membrane bound protein that also has an active nuclear-translocation component that can alter specific gene expression and cell proliferation. Procadherin FAT1 is important in cell polarization and directed cell migration and also modulates cell-cell contact interactions. Procadherin FAT1 is expressed in epithelial, endothelial and smooth muscle cells and plays roles in their response to injury and is often highly induced after arterial injury and effects vascular smooth muscle cell growth and migration. Procadherin FAT1 is also important in cancer where it appears to function as both an oncogene and a tumor suppressor depending upon the cell context. Also recent research has shown that soluble FAT1 can be a novel biomarker candidate for such cancers as pancreatic cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Procadherin FAT1 Antibody, clone C257 is validated for use in Western Blotting and Immunoprecipitation for the detection of Procadherin FAT1. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MV3 cell lysate. Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Procadherin FAT1 in 10 µg of FAT1 transfected MDA-MB-231 and MDA-MB-468 cell lysate. Immunoprecipitation Analysis: A representative lot immunoprecipitated Procadherin FAT1 in C257 cell lysate. (Courtesy of Dr. R. Thorne, U of Newcastle, AU.) |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to Human FAT1. |
| Clone | C257 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | >260 kDa observed. Procadherin FAT1 is an extremely large cell surface receptor with a calculated MW of 506 kDa and its highly glycosolated. It may be difficult to resolve the protein by western blot. An uncharacterized band at 50 kDa and other lower MWs may be observed in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in FAT1 transfected NIH-3T3 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Procadherin FAT1 in 10 µg of FAT1 transfected NIH-3T3 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |