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Millipore Anti-S100a4 Antibody, Clone 9d5.1 Is An Antibody Against S100a4 For Use In Western Blotting, Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Protein S100-A4 (UniProt P26447; also known as Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4) is encoded by the S100A4 (also known as CAPL, MTS1, P9KA, PEL98) gene (Gene ID 6275) in human. S100A4 belongs to a family of low moleclar weight proteins characterized by two calcium-binding helix-loop-helix EF-hand domains (a.a. 12-47 & 50-85 of human S100A4). S100 proteins exist as non-covalent homodimers with cell-specific expression patterns. S100A4 associates with cytoskeletal proteins, such as myosin and F-actin, as well as other actin-binding proteins such as Talin and integrins, and is involved in controlling not just shape and motility of normal cells, but also plays a role in cancer cells invasion/metastasis.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-S100A4 Antibody, clone 9D5.1 is an antibody against S100A4 for use in Western Blotting, Immunocytochemistry.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100A4 in HUVECs, HeLa, A431, and NIH/3T3 cells.
Biological Information
Immunogen
GST-tagged recombinant human S100A4 internal fragment.
Epitope
Within EF-hands 1&2.
Clone
9D5.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 9D5.1 was raised against human S100-A4 internal fragment that shares high sequence homology with the corresponding region in mouse (96%) and rat (93%) protein.
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002952
Gene Symbol
S100A4
CAPL
MTS1
P9KA
PEL98
Purification Method
Protein G Purified
UniProt Number
P26447
Molecular Weight
~12 kDa oberved. 11.73/11.72/11.78 kDa (human/mouse/rat) calculated. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
