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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC653 |
| Description | Anti-S100A4 Antibody, clone 9D5.1 |
| Alternate Names |
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| Background Information | Protein S100-A4 (UniProt P26447; also known as Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4) is encoded by the S100A4 (also known as CAPL, MTS1, P9KA, PEL98) gene (Gene ID 6275) in human. S100A4 belongs to a family of low moleclar weight proteins characterized by two calcium-binding helix-loop-helix EF-hand domains (a.a. 12-47 & 50-85 of human S100A4). S100 proteins exist as non-covalent homodimers with cell-specific expression patterns. S100A4 associates with cytoskeletal proteins, such as myosin and F-actin, as well as other actin-binding proteins such as Talin and integrins, and is involved in controlling not just shape and motility of normal cells, but also plays a role in cancer cells invasion/metastasis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-S100A4 Antibody, clone 9D5.1 is an antibody against S100A4 for use in Western Blotting, Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100A4 in HUVECs, HeLa, A431, and NIH/3T3 cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human S100A4 internal fragment. |
| Epitope | Within EF-hands 1&2. |
| Clone | 9D5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 9D5.1 was raised against human S100-A4 internal fragment that shares high sequence homology with the corresponding region in mouse (96%) and rat (93%) protein. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~12 kDa oberved. 11.73/11.72/11.78 kDa (human/mouse/rat) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |