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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IF, IHC, FC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC667 |
| Description | Anti-GPC3 Antibody, clone 9C2 |
| Alternate Names |
|
| Background Information | Glypican-3 or GTR2-2 is the protein product of the gene GPC3. It is a cell surface heparin sulfate containing glycoprotein that plays an important role in the modulation of growth and development of mesodermal tissues and organs, as well as various cancers. Among its many known functions, it is a regulator of the dipeptidyl peptidase DPP4 (CD26). DPP4 plays an important role in glucose metabolism via its action on incretin and GLP-1 hormones. DPP4 also is a cancer marker and thought to function as a cancer suppressor. Thus acting as a regulator of DPP4, Glypican-3 (GPC3) participates in everything from glucose and blood sugar regulation to tumor growth. Glypican-3 (GPC3) is most highly expressed in the lung, liver and kidney, and typically a secreted form, secreted glypican-3, is found in the extracellular space generated as a cleavage product by the action of a convertase. Defects in GPC3 cause Simpson-Golabi-Behmel syndrome type 1 (SGBS1) or Simpson dysmorphia syndrome (SDYS). This is a condition characterized by pre-and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. EMD-Millipore’s anti-GPC3 mouse monoclonal antibody, clone 9C2, has been tested in western blot against recombinant human protein, human HepG2, HEK293, Jurkat, and SK-N-SH cell line lysates, as well as rat PC-12 and mouse liver cell lysates. In addition, the clone as been successfully used in paraffin-embedded immunohistochemistry on human liver and breast cancer tissues and in immunofluorescent immunocytochemistry on human HeLa cells in culture. Finally, the clone has been tested successfully on human cells by flow cytometry and against the recombinant antigen by ELISA. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Purified mouse monoclonal in PBS with 0.05% sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Glypican using this mouse monoclonal antibody, Anti-GPC3 Antibody, clone 9C2 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GPC3 in human liver and kidney tissues. Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GPC3 in HeLa cells. Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected GPC3 in Jurkat cells. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified recombinant fragment of human GPC3 expressed in E. Coli. |
| Clone | 9C2 |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~66 kDa observed. Uncharacterized bands may appear in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates. Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPC3 in mouse liver, HepG2, HEK293, Jurkat, SK-N-SH, PC-12, and F9 cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |