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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, ICC, ChIP | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABD20 |
| Description | Anti-PAX7 Antibody, clone 2F12H4 |
| Alternate Names |
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| Background Information | Paired box protein Pax-7 (PAX7) belongs to the Pax family of transcription factors, a highly conserved group of proteins with crucial roles in tissue homeostasis and organogenesis. PAX7 is involved in the specification, survival, maintenance, and self-renewal of skeletal muscle progenitors and myogenic progenitors. It is primarily expressed in newly activated and quiescent satellite cells. Studies have shown that on activation, quiescent satellite cells co-express MyoD with PAX7 prior to proliferation, after which one or the other transcription factor is downregulated. Downregulation of MyoD instead of PAX7 leads to withdrawal from the cell cycle and myogenic differentiation, causing the cell to return to seemingly quiescent state. Chromosomal aberrations relating to PAX7 have been linked to rhabdomysarcoma 2 (RMS2). |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-PAX7 Antibody, clone 2F12H4 is a Rat monoclonal antibody for detection of PAX7 also known as paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7 & has been validated in WB, ICC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry: A previous lot of this antibody was shown to detect Pax7 localized in euchromatin of human Ewing’s sarcoma cells. The antibodies are detected with Alexa Fluor® 488-conjugated anti-rat IgG. Each sample is counterstained with hoechst (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to mouse PAX7. |
| Clone | 2F12H4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with mouse. Shown to react with human by an independent laboratory (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039). |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: Probable transcription factor. May have a role in myogenesis. SUBUNIT STRUCTURE: Can bind to DNA as a heterodimer with PAX3. Interacts with DAXX (By similarity). SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: Expressed specifically during the development of the nervous and muscular system. SEQUENCE SIMILARITIES: Belongs to the paired homeobox family. Contains 1 homeobox DNA-binding domain. Contains 1 paired domain. |
| Molecular Weight | ~ 55 kDa observed. The calculated MW of PAX7 is 55 kDa; however, expression of PAX7 can have multiple isoforms. (Robert B., et al. (2008). Physiol. Genomics 33:41-49.) |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse fetal brain tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PAX7 in 10 µg of mouse fetal brain tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |