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NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors. NANOG has also been identified as a key transcription factor used to generate induced pluripotent stem cells.
Product Information
Format
AlexaFluor®488
Control
H9 human embryonic stem cells.
Presentation
Purified mouse monoclonal IgG2a conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.
GST-tagged recombinant protein corresponding to human Nanog
Clone
7F7.1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_079141
Gene Symbol
NANOG
Purification Method
Protein G Purified
UniProt Number
Q9H9S0
Molecular Weight
The uncojugated parent antibody (Catalog No. MABD24) has an observed MW of 39 kDA.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected NANOG in H9 human embryonic stem cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
