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Millipore Detect Hla-Drb1 Using This Anti-Hla-Drb1 Antibody, Clone Dj9 Validated For Use In Western Blotting, Icc, Ihc, Flow Cytometry. - Mill (Additional S&H or Hazmat Fees May Apply)
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells and presents them on the cell surface for recognition by the CD4 T-cells. Class II molecules are expressed in antigen presenting cells B lymphocytes, dendritic cells, macrophages. HLA-Drb1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. HLA-DRB1, clone DJ9, has been shown to be a relevant marker of bone marrow derived mesenchymal stem cells and their osteogenic derivatives (Andersen, D., et al., 2011).
Product Information
Format
Purified
Control
Daudi cell lysates
Presentation
Purified mouse monoclonal IgG2aλ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect HLA-DRB1 using this Anti-HLA-DRB1 Antibody, clone DJ9 validated for use in Western Blotting, ICC, IHC, Flow Cytometry.
Key Applications
Western Blotting
Immunocytochemistry
Immunohistochemistry
Flow Cytometry
Application Notes
This antibody has been shown to work in Immunocytochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Immunohistochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Flow Cytometry (Andersen, D., et al. (2011)).
Biological Information
Immunogen
Whole hMSC-TERT Cells
Clone
DJ9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes human HLA-DRB1.
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002112
Gene Symbol
HLA-DRB1
Purification Method
Protein G Purified
UniProt Number
Q5Y7A7
Molecular Weight
~30 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in Daudi cell lysates. Western Blot Analysis: A 1:100,000 dilution of this antibody detected HLA-DRB1 in Duadi cell lysates.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
