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Millipore Anti-Trrap Antibody, Clone 2trr-1b3 Is An Antibody Against Trrap For Use In Western Blotting, Immunoprecipitation. - Mill (Additional S&H or Hazmat Fees May Apply)
Transformation/transcription domain-associated protein
350/400 kDa PCAF-associated factor
PAF350/400
STAF40
TR-AP
Tra1 homolog
Background Information
Transformation/transcription domain-associated protein (UniProt Q9Y4A5; also known as 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog) is encoded by the TRRAP (also known as PAF400) gene (Gene ID 8295) in human. Transformation/transcription domain-associated protein (TRRAP) is a cofactor component shared by several histone acetyltransferase (HAT) complexes, including SAGA (Spt-Ada-Gcn5-acetyltransferase), PCAF (P300/CBP-associated factor), and Tip60. TRRAP is an essential component of the histone epigenetic modifying mechinery that plays an important role in regulating cell-cycle progression. Trrap gene-knockdown or protein downregulation causes cell and mouse lethality, impaired spindle checkpoint and differentiation.
Product Information
Format
Ascites
Presentation
Mouse monoclonal IgG2aκ ascites without preservatives.
Quality Level
MQ100
Applications
Application
Anti-TRRAP Antibody, clone 2TRR-1B3 is an antibody against TRRAP for use in Western Blotting, Immunoprecipitation.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated TRRAP from 1 mL (4.5 mg/mL) of HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France). Immunoprecipitation Analysis: A representative lot immunodepleted TRRAP using nonhomologous end-joining/NHEJ-competent protein extracts prepared from human AAH-1 lymphoblastoid cells (Robert, F., et al. (2006). Mol. Cell. Biol. 26(2):402-412).
Biological Information
Immunogen
Ovalbumin-conjugated linear peptide corresponding to a sequence from the TP53-interacting region of human TRRAP.
Epitope
N-terminal to nuclear localization signal in the TP53-interacting region.
Clone
2TRR-1B3
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
The immunogen sequence is present in both spliced isoforms of human TRRAP reported by UniProt (Q9Y4A5).
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001231509
Gene Symbol
TRRAP
PAF400
Purification Method
Unpurified
UniProt Number
Q9Y4A5
Molecular Weight
~400 kDa observed. 437.6 kDa (isoform 1) and 434.4 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evalulated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRRAP in 10 µg of HeLa nuclear extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
