Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, ICC, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1116 |
| Description | Anti-DNAJB1/Hdj1 Antibody, clone J25 |
| Alternate Names |
|
| Background Information | DnaJ homolog subfamily B member 1 (UniProt Q3MI00) is encoded by the DNAJB1 gene (Gene ID 538426) in bovine species. Heat shock proteins (Hsps) are classified into six major families based on their relative molecular mass, Hsp110/hspH, Hsp90/HspC, Hsp70/HspA, Hsp60/HspD, Hsp40/DnaJ, and small heat shock proteins sHsp/HspB. Hsp40/DnaJB1 belongs to the Hsp40/DnaJ subfamily of heat shock proteins. Hsp40 acts as a co-chaperone with Hsp70 to facilitate protein folding, transport, and degradation. MK5 is reported to stimulate the ATP hydrolyse activity of Hsp40/Hsp70 complex by phosphorylating Hsp40/DnaJB1 C-terminal serine residues Ser149, Ser151, Ser171, resulting in an enhancement of Hsp40/DnaJB1-dependent suppression of heat shock factor 1-driven transcription. DnaJB1 is also shown to interact with MDM2 and prevent MDM2-mediated ubiquitination and proteasomal degradation of p53. DnaJB1 depletion promotes cancer cell growth as a result of p53 pathway inactivation. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-DNAJB1/Hdj1 Antibody, clone J25 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of DNAJB1/Hdj1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences). Flow Cytometry Analysis: 1-5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences). Western Blotting Analysis: 0.05-0.5 µg/mL from a representative lot detected heat shock-induced DNAJB1 upreguation in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroleukemia cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences). |
| Biological Information | |
|---|---|
| Immunogen | Purified bovine DNAJB1. |
| Clone | J25 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Bovine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~38 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human K562 erythroblastoma cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected heat shock-induced DNAJB1/Hdj-1 upregulation in human K-562 erythroleukemia cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |