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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IP, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE126 |
| Description | Anti-SR protein family Antibody, clone 16H3 |
| Alternate Names |
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| Background Information | Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including the identification and appropriation of splice-sites and the manipulation of alternative splicing regulation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SR protein family Antibody, clone 16H3 is a Mouse Monoclonal Antibody for detection of SR protein family also known as arginine/serine-rich splicing factor 6 & has been validated in WB, IP, ICC. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Neugebauer, KM, et al. (1995). Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Neugebauer, KM, et al. (1995). |
| Biological Information | |
|---|---|
| Immunogen | Dephosphorylated bovine SRp55 and GST-tagged recombinant dephosphorylated SRp55 (Neugebauer, KM et. al 1995). |
| Clone | 16H3 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes the four nuclear proteins SRp75, SRp55, SRp40, and SRp20, plus ~20 other prominent nuclear proteins believed to correspond to the SR family. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants. |
| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. SUBUNIT STRUCTURE: Binds SFRS12. SUBCELLULAR LOCATION: Nucleus. PTM: Extensively phosphorylated on serine residues in the RS domain (By similarity). SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family. Contains 2 RRM (RNA recognition motif) domains. |
| Molecular Weight | Multiple SR protein family members detected. Reported to predominantly detect SRp75, SRp55, SRp40, SRp30, SRp20 (Neugebauer, KM, et al. (1995). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HeLa nuclear extract. Western Blot Analysis: 0.5 µg/mL of this antibody detected SR proteins in 10 µg of HeLa nuclear extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |