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Millipore This Anti-Kdm5b Antibody, Clone 15g8.1 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Kdm5b. - Mill (Additional S&H or Hazmat Fees May Apply)
Lysine-specific demethylase 5B (UniProt Q9UGL1; also known as Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji AT rich interactive domain 1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Protein phosphatase 1 regulatory subunit 98, Putative DNA/chromatin binding motif, RBP2-H1, Retinoblastoma-binding protein 2 homolog 1) is encoded by the KDM5B (also known as PLU1, PUT1, JARID1B, PPP1R98, RBBP2H1, RBBP2H1A) gene (Gene ID 10765) in human. JARID1B/KDM5B is an H3K4me2/3 demethylase that regulates self-renewal and differentiation in a cell type-specific manner. JARID1B/KDM5B is highly expressed in embryonic stem cells (ESCs), hematopoietic stem cells (HSCs), and in various primary cancers including melanoma, breast, testicular, and is upregulated in leukemic cell lines.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-KDM5B Antibody, clone 15G8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of KDM5B.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected KDM5B in 10 µg of MCF-7 cell lysate. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KDM5B in human kidney and mouse cerebral cortex tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to the region between ARID and JmjC domain of human KDM5B.
Epitope
Region between ARID and JmjC domain
Clone
15G8.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Expected to react with both spliced isoforms.
Isotype
IgG2aκ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_006609
Gene Symbol
KDM5B
JARID1B
PLU1
RBBP2H1
Purification Method
Protein G Purified
UniProt Number
Q9UGL1
Molecular Weight
~175 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected KDM5B in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
