The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Use Anti-Srp55 Antibody, Clone 9-1-56 (Mouse Monoclonal Antibody) Validated In Wb, Ip, Icc, Ihc To Detect Srp55 Also Known As Arginine/Serine-Rich Splicing Factor 6, Pre-Mrna-Splicing - Mill (Additional S&H or Hazmat Fees May Apply)
Pre-mRNA-splicing factor SRp55 is a member of the serine/arginine-rich (SR) protein family. This group of proteins is found mainly in the nucleus where they play a critical role in regulated and constitutive splicing of precursor mRNAs. SRp55 is involved in the constitutive process for mRNA splicing, and is able to mediate alternative splice site selection. Considered one of the more ubiquitous of the splice factors, SRp55 is upregulated in response to DNA damage when p53 is lacking. SRp55 is significantly phosphorylated on RS domain serine residues.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Use Anti-SRp55 Antibody, clone 9-1-56 (Mouse Monoclonal Antibody) validated in WB, IP, ICC, IHC to detect SRp55 also known as arginine/serine-rich splicing factor 6, Pre-mRNA-splicing factor SRP55.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Biological Information
Immunogen
MBP-tagged recombinant protein corresponding to human SRp55.
Epitope
Not determined
Clone
9-1-56
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_006266
Entrez Gene Summary
The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants.
Gene Symbol
SRP55
SFRS6
B52
Purification Method
Protein G
UniProt Number
Q13247
UniProt Summary
FUNCTION: Plays a role in constitutive splicing and can modulate the selection of alternative splice sites.
SUBUNIT STRUCTURE: Binds SFRS12.
SUBCELLULAR LOCATION: Nucleus.
PTM: Extensively phosphorylated on serine residues in the RS domain (By similarity).
SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.
Contains 2 RRM (RNA recognition motif) domains.
Molecular Weight
~ 43 kDa observed. 40 kDa calculated.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected SRp55 on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
