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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE1529 |
| Replaces | MABE66 |
| Description | Anti-BRD7 Antibody, clone BRM 2D3 |
| Alternate Names |
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| Background Information | Bromodomain-containing protein 7 (UniProt Q9NPI1; also known as 75 kDa bromodomain protein, Protein CELTIX-1) is encoded by the BRD7 (also known as BP75, CELTIX1) gene (Gene ID 29117) in human. BRD7 is a ubiquitously expressed single bromodomain-/BRD-containing member of the family of BRD-containing proteins. BRD7 functions as a subunit of the SWI/SNF chromatin-remodeling complex involved in transcription regulation. It also interacts with the well known tumor suppressor p53 to trans-activate genes involved in cell cycle arrest. BRD7 is downregulated in colorectal carcinoma and nasopharyngeal carcinoma, and BRD7 overexpression is reported to inhibit nasopharyngeal carcinoma cell proliferation via cell cycle arrest by transcriptionally regulating ras/MEK/ERK, Rb/E2F, b-catenin and ERK pathways. BRD7 mRNA has been identified as a silencing target of miR-300, overexpression of miR-300 promotes the proliferation, invasion and EMT of osteosarcoma cells through targeting BRD7 mRNA. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-BRD7 Antibody, clone BRM 2D3 is validated for use in Western Blotting, Immunoprecipitation for the detection of BRD7. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected BRD7 in 10 µg of HEK293 and HeLa cell lysates. Immunoprecipitation Analysis: A representative lot immunoprecipitated BRD7 from H1299 cell lysate (Courtesy of Thomas Dobner, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg, D-93053 Regensburg, Germany). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human BRD7. |
| Clone | BRM203 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone BRM 2D3 detects human 75 kDa bromodomain protein, also known as protein CELTIX-1. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~75 kDa observed. 74.14 kDa (isoform 1), 74.27 kDa (isoform 2) calculated. Added Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected BRD7 in 10 µg of Jurkat cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |