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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE163 |
| Replaces | MABE512 |
| Description | Anti-ASF-1 (SF2) Antibody, clone AK96 |
| Alternate Names |
|
| Background Information | Alternative-splicing factor 1 (ASF-1), also known as SRp30a or SF2, is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-ASF-1 (SF2) Antibody, clone AK96 (mouse monoclonal antibody) validated in WB, IP, ICC, IHC to detect ASF-1 (SF2) also known as splicing factor arginine/serine-rich 1, Alternative-splicing factor 1 & pre-mRNA-splicing factor SF2 P33 subunit |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ASF-1 (SF2) in human prostate carcinoma cells and human testis tissue. Western Blotting Analysis: A representative lot was used by an independent laboratory in western blotting (Sinha, R., et al. (2010). Mol. Cell. Biol. doi:10.1128/MCB.01270-09). Immunoprecipitation Analysis: A representative lot immunoprecipitated ASF-1 (SF2) in HeLa nuclear extracts (Jablonski, J. A., et al. (2010). Nucleic Acids Research. 38(4):1249-1260). |
| Biological Information | |
|---|---|
| Immunogen | MBP-tagged recombinant protein corresponding to the N-terminus of human ASF-1 (SF2). |
| Epitope | N-terminus |
| Clone | AK96 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes the N-terminus of ASF-1 (SF2). |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Human. Immunogen sequence is identical in mouse and rat, so reactivity is predicted. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The proteins ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~ 30 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in human lymph node lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected ASF-1 (SF2) on 10 µg of human lymph node lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |