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Millipore Use Anti-Lrh-1 Antibody, Clone 1lrh-2e1 (Mouse Monoclonal Antibody) Validated In Wb, Icc To Detect Lrh-1 Also Known As Liver Receptor Homolog 1. - Mill (Additional S&H or Hazmat Fees May Apply)
Liver receptor homolog 1 (LRH-1, CPF, FTF, PHR-1, FTF, or hB1F) is an orphan nuclear receptor that is highly expressed in the intestines, pancreas, ovary, and liver. LRH-1 is a constitutively active transcription factor with a complex role in many cellular processes which may include lipid metabolism, cell proliferation, development, reproduction, and immunity. LRH-1 may interact with an array of transcriptional coactivators that modulate its activity, among which are the MBF1, SRC, and PGC-1α proteins. In addition, ERK phosphorylation on Ser-238 and Ser-243 also enhances LRH-1 activity. However, LRH-1 is also negatively regulated by several factors. For instance, corepressors such as SHP, DAX-1, and PROX-1, preclude its binding to DNA; and sumoylation promotes its inactivation and sequestration into nuclear bodies. LRH-1 targets many genes including the CYP7A1 and CYP8B1 genes involved in bile synthesis; and Oct4 required for pluripotency in embryonic stem cells. LRH-1 has been implicated in inflammatory bowel disease, and tumor development in the intestines and pancreas.
Product Information
Format
Ascites
Control
Mouse pancreas tissue lysate
Presentation
Mouse monoclonal IgG2aλ ascites with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Use Anti-LRH-1 Antibody, clone 1LRH-2E1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect LRH-1 also known as Liver receptor homolog 1.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Western Blot Analysis: A 1:1,000 dilution from representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cell lysates (M. Oulad, IGBMC, France.).
Immunocytochemistry Analysis: A representative lot detected Liver receptor homolog-1 in HepG2 and HeLa cells (M. Oulad, IGBMC, France.).
Biological Information
Immunogen
Linear peptide corresponding to the mouse Liver receptor homolog-1.
Clone
1LRH-2E1
Host
Mouse
Species Reactivity
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_109601
Gene Symbol
Nr5a2
Lrh1
Purification Method
Unpurified
UniProt Number
P45448
Molecular Weight
~64 kDa, ~56 kDa, and ~42 kDa observed. This antibody detects three isoforms of Liver receptor homolog-1 as described by Uniprot No. O00482.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse pancreas tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Liver receptor homolog-1 in 10 µg of mouse pancrease tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
