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Millipore This Anti-Jmjd2a Antibody, Clone 8b7.1 Is Validated For Use In Western Blotting, Ihc For The Detection Of Jmjd2a. - Mill (Additional S&H or Hazmat Fees May Apply)
JmjC domain-containing histone demethylation protein 3A
Jumonji domain-containing protein 2A
Background Information
Jumonji domain-containing protein 2A (JMJD2A), or KDM4A, is a ubiquitously expressed demethylase enzyme, which belongs to the JMJD2 family. Like other members of this family, JMJD2A contains multiple domains: two Tudor domains, two PHD-type zinc fingers, one JmjC domain, and one JmjN domain. Little is known about the function of most of these domains; however, they are believed to be involved in transcriptional regulation. JMJD2A functions as a histone demethylase with specific activity against trimethylated Histone H3 Lysine residues Lys-9 and Lys-36. It has no effect on any other lysine residues, or on mono- and dimethylated Lys-9 and Lys-36. The demethylation of these residues induces the production of succinate and formaldehyde. Studies have shown that JMJD2A recruits NCOR1 and histone deacetylases, causing the respective transcriptional repression of E2Fand ASCL2 responsive promoters.
Product Information
Format
Purified
Control
U2OS nuclear extract
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-JMJD2A Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of JMJD2A.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected JMJD2A in human colorectal adenocarcinoma cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human JMJD2A.
Clone
8B7.1
Host
Rabbit
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_055478
Gene Symbol
KDM4A
JHDM3A
JMJD2
KIAA0677
Purification Method
Protein G Purified
UniProt Number
O75164
Molecular Weight
~120 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in U2OS nuclear extract.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected JMJD2A in 10 µg of U2OS nuclear extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
