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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, DB, ICC, IP, IHC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE241 |
| Description | Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12 |
| Alternate Names |
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| Background Information | Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Conversely,deacetylation of histone H3 lysine 9 is associated with gene-activating events. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Mouse monoclonal IgG1κ ascites with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12 is a Mouse Monoclonal Antibody for detection of dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) also known as Histone H3.1 & has been validated in WB, DB, ICC, IP & IHC. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A representative lot was used by an independent laboratory in KCl whole-cell extract. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.) Dot Blot Analysis: A representative lot was used by an independent laboratory in unmodified and modified Histone H3 peptides. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.) Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in NIH/3T3 cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.) Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in mouse testis tissue. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.) Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.) |
| Biological Information | |
|---|---|
| Immunogen | Ovalbumin-conjugated linear peptide corresponding to human Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28. |
| Epitope | Dimethylated and phosphorylated at Lys9/27 and Ser10/28. |
| Clone | 51TA-2H1 |
| Host | Mouse |
| Specificity | This antibody recognizes Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28. |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in untreated and etoposide acid extract treated HeLa cell lysates. Western Blot Analysis: A 1:5,000 dilution of this antibody detected Histone H3 on 10 µg of untreated and etoposide acid extract treated HeLa cell lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |