The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Use Anti-Ago2 Antibody, Clone 11a9 (Rat Monoclonal Antibody) Validated In Wb, Ip, Icc To Detect Ago2 Also Known As Protein Argonaute-2, Argonaute2, Hago2, Eif-2c 2. - Mill (Additional S&H or Hazmat Fees May Apply)
Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Use Anti-Ago2 Antibody, clone 11A9 (Rat Monoclonal Antibody) validated in WB, IP, ICC to detect Ago2 also known as Protein argonaute-2, Argonaute2, hAgo2, eIF-2C 2.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Ago2 in HEK239 cell lysate (Rudel, S., et al. (2008) RNA. 14(6):1244-1253.).
Immunocytochemistry Analysis: A represeentative lot from an independent laboratory detected Ago2 in HEK293, HeLa, and RPE-1 cells (Weinmann, L., et al. (2009). Cell. 136(3):496-507.).
Biological Information
Immunogen
Linear peptide corresponding to human Ago2.
Clone
11A9
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rat
Isotype
IgG2aκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001158095
Gene Symbol
EIF2C2
AGO2
Purification Method
Protein G Purified
UniProt Number
Q9UKV8
Molecular Weight
~85 kDa observed. Two isoforms at ~97 kDa and ~94 kDa are known to exist.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected Ago2 in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
