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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IP, ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE254 |
| Description | Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 |
| Alternate Names |
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| Background Information | Protein-arginine deiminase type-4 (PAD4; formerly PAD5) is one of five PAD (1-4, 6) homologues that belong to the superfamily of multidomain amidinotransferases. The PAD enzymes catalyze deiminating reactions in which guanidinium is hydrolyzed to peptidylcitrulline. PAD4 is a calcium-regulated nuclear-localized enzyme. It modifies arginine residues on histones H2A, H3, and H4 (H2 Arg3; H3 Arg2, H3 Arg8, H3 Arg17, H3 Arg26; and H4 Arg3). Several studies have suggested that PAD4-mediated citrullination has important implications for gene regulation. PAD4 may perform deimination at sites overlapping with arginine methylation, and antagonize the transcription-promoting effects of methylating enzymes such as CARM1 and PRMT1. PAD4 may therefore inhibit gene expression, most notably expression of cell-cycle regulating p53 genes such as p21. Previous studies have suggested that PAD4 may play central role in the development of cancer. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
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| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 (mouse monoclonal antibody) validated in WB, IP to detect PAD-4 also known as HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated Protein-arginine deminiase 4 in IP (Prof. K. Mowen, Department of Chemical Physiology, The Scripps Research Institute-California Campus.). Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Protein-arginine deminiase 4 in ChIP-Seq (Price, D. H., et al. (2011). Mol Cell. 45(1):38-50.). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Protein-arginine deminiase 4. |
| Clone | 10F1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~74 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in DMSO treated and untreated HL-60 cell lysates. Western Blot Analysis: 0.25 µg/mL of this antibody detected Protein-arginine deminiase 4 in 10 µg DMSO treated and untreated HL-60 cell lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |