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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IP, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE283 |
| Description | Anti-p53 Antibody, clone PAb421 |
| Alternate Names |
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| Background Information | p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kDa phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy. Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features: Most of them are missense point mutations giving rise to an altered protein function. Also, many, but not all, mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Use Anti-p53 Antibody, clone PAb421 (Mouse Monoclonal Antibody) validated in WB, IP, IHC to detect p53 also known as Cellular tumor antigen p53, Tumor suppressor p53. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot from independent laboratory immunoprecipitated p53 in IP (Lehman, T. A., et al. (1991). Cancer Res. 51(15):4090-4096.; Harlow, E., et al. (1981). J Virol. 39(3):861-869.) Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in human breast cancer tissue (Davidoff, A. M., et al. (1992). Proc Natl Acad Sci USA. 89(8):3439-3442.). |
| Biological Information | |
|---|---|
| Immunogen | Partially purified mouse p53 |
| Epitope | Amino acids 376-378 in human p53 |
| Clone | PAb421 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes amino acids 376-378 of human p53. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse. Demonstrated to react with Human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~53 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain tissue lysate. Western Blot Analysis: 1 µg/mL of this antibody detected p53 in 10 µg of mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |