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Millipore Anti-Dnmt3b Antibody, Clone D3b2-2c1 Is A Rat Monoclonal Antibody For Detection Of Dnmt3b Also Known As Dna (Cytosine-5)-Methyltransferase 3b & Has Been Validated In Wb & Ip. - Mill (Additional S&H or Hazmat Fees May Apply)
DNA (cytosine-5)-methyltransferase 3B (Dnmt3B) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. The Dnmt3b enzyme is involved in de novo methylation and the establishment of genome-wide DNA methylation patterns during development. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.
Product Information
Format
Purified
Control
HeLa nuclear extract
Presentation
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Dnmt3b Antibody, clone D3B2-2C1 is a Rat Monoclonal Antibody for detection of Dnmt3b also known as DNA (cytosine-5)-methyltransferase 3B & has been validated in WB & IP.
Key Applications
Western Blotting
Immunoprecipitation
Application Notes
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt3b in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
Biological Information
Immunogen
Linear peptide corresponding to human Dnmt3b.
Clone
D3B2-2C1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rat
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001193984
Gene Symbol
DNMT3B
Purification Method
Protein G Purified
UniProt Number
Q9UBC3
Molecular Weight
~110 kDa observed. Multiple isoforms between ~80-96 kDa are known to exist. A truncated protein at ~40 kDa may be observed in some cell lysates (Ostler, K. R., et al. (2007). 26(38): 5553-5563.). Uncharacterized bands may be observed at ~20 kDa and ~34 kDa in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Dnmt3b in 10 µg of of HeLa nuclear extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
