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Millipore Anti-Dnmt1 Antibody, Clone Dnm-2c1 Is A Rat Monoclonal Antibody For Detection Of Dnmt1 Also Known As Dna (Cytosine-5)-Methyltransferase 1 & Has Been Validated In Ip, Chip & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
DNA (cytosine-5)-methyltransferase 1 (Dnmt1) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. These methylation marks are heritable modifications that are transferred to new DNA molecules following replication, by the activity of Dnmt1, and possibly Dnmt3a and Dnmt3b. Dnmt1 recognizes hemimethylated CpG regions with the aid of the UHRF1 protein, and it mediates the methylation of cytosines at those sites. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.
Product Information
Format
Purified
Control
HeLa nuclear extract
Presentation
Purified rat monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Dnmt1 Antibody, clone DNM-2C1 is a Rat Monoclonal Antibody for detection of Dnmt1 also known as DNA (cytosine-5)-methyltransferase 1 & has been validated in IP, ChIP & WB.
Key Applications
Immunoprecipitation
Western Blotting
Chromatin Immunoprecipitation (ChIP)
Application Notes
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect Dnmt1 in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).
Biological Information
Immunogen
His-tagged recombinant protein corresponding to human Dnmt1.
Clone
DNM-2C1
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rat
Isotype
IgG2b
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001124295
Gene Symbol
DNMT1
AIM
CXXC9
DNMT
Purification Method
Protein G Purified
UniProt Number
P26358
Molecular Weight
~183 kDa observed. Three isoforms are known to exist at ~183 kDa, ~185 kDa, and ~145 kDa. An uncharacterized band may be observed at ~90 kDa in some nuclear extracts.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.02 µg/mL of this antibody detected Dnmt1 in 10 µg of HeLa nuclear extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
