Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE325 |
| Description | Anti-p21WAF1 Antibody, clone EA10 |
| Alternate Names |
|
| Background Information | The tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The ~21 kDa product of the WAF1 gene is found in a complex involving cyclins, CDKs, and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is the prevention of CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. While the role of p21WAF1 in apoptosis is less clear, it is known that p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 can occur by both p53-dependent and p53-independent mechanisms, in response to certain observed conditions. p21WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Its overexpression was observed to inhibit cellular growth. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-p21WAF1 Antibody, clone EA10 is a Mouse Monoclonal Antibody for detection of p21WAF1 & has been validated in WB, FC. |
| Key Applications |
|
| Application Notes | Western Blot Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428). Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p21WAF1 in normal human lower back tissue (el-Deiry, W. S., et al. (1995). Cancer Res. 55(13):2910-2919.). Flow Cytometry Analysis: A representative lot from an independent laboratory detected p21WAF1 in non-irradiated and gamma irradiated CLL cells (Carter, A., et al. (2004). Br J Haematol. 127(4):425-428). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein corresponding to human p21WAF1. |
| Clone | EA10 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~21 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in HT-29 cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected p21WAF1 in 10 µg of HT-29 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |