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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, Porcine, R | WB, IP, ChIP, ICC, IHC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE328 |
| Description | Anti-MeCP2 Antibody, clone 4H7 |
| Alternate Names |
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| Background Information | DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA. It represses transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This MeCP2 antibody is validated for use in WB, IHC, ICC, ChIP & IP for the detection of the MeCP2 protein |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse whole brain cell lysates (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). Chromatin Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 knockout brain nuclear extracts (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). Immunocytochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, GFP-meCP2 transfected C2C12 cells (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect MeCP2 in various, mouse WT, but not in MeCP2 hemizygous null brain tissue sections (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). |
| Biological Information | |
|---|---|
| Immunogen | Full length, strep-tagged N-terminus of rat MeCP2. |
| Epitope | C-terminus |
| Clone | 4H7 |
| Host | Rat |
| Specificity | This antibody recognizes the C-terminus of MeCP2, which has been epitope mapped between amino acids 310-492. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Demonstrated to react with Mouse. Predicted to react with Porcine and Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~75 kDa observed. Uniprot describes an isoform produced by alternative splicing at ~55 kDa. The calculated molecular weight is 53 kDa, however MeCP2 has been observed between ~75-100 kDa in western blots (Jost, K. L., et al. (2011). PLoS ONE. 6(11):e26499). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blot in mouse brain tissue nuclear extract. Western Blot Analysis: A 1:1,000 dilution of this antibody detected MeCP2 in 10 µg of mouse brain tissue nuclear extract. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |