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Millipore Anti-C-Fos Antibody, Clone 2g9c3 Is A Mouse Monoclonal Antibody For Detection Of C-Fos Also Known As Proto-Oncogene C-Fos, Cellular Oncogene Fos & Has Been Validated In Wb, Icc. - Mill (Additional S&H or Hazmat Fees May Apply)
c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.
Product Information
Format
Purified
Control
HeLa cell lysate
Presentation
Purified mouse monoclonal IgG1κ from cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-c-Fos Antibody, clone 2G9C3 is a Mouse Monoclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB, ICC.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A representative lot detected c-Fos in schwann cells. (Lafarga, M., et al. (2002). Molecular Biology of the Cell. 13:2771–2782.)
Biological Information
Immunogen
Linear peptide corresponding to human c-Fos.
Clone
2G9C3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins.
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_005243
Gene Symbol
FOS
G0S7
c-Fos
Purification Method
Protein G Purified
UniProt Number
P01100
Molecular Weight
~55/62 kDa observed. This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. The calculated molecular wieght is 46 kDa; however, c-Fos has been observed at ~55 kDa in western blots. (Assim, M., et al. (2010). Journal of Biological Chemistry. 285(26):20343–20357.)
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos on 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
