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Millipore Anti-C-Erbb2/C-Neu Antibody, Clone 3b5 Is A Mouse Monoclonal Antibody For Detection Of C-Erbb2/C-Neu Also Known As Mln 19, Proto-Oncogene Neu, Proto-Oncogene C-Erbb-2 Or - Mill (Additional S&H or Hazmat Fees May Apply)
The ErbB2 (HER2, c-Neu, ErbB2, c-erbB2, or HER2/neu) protein is member of the human epidermal growth factor receptor (HER) family. It is a receptor tyrosine kinase containing an intracellular kinase domain and an extracellular ligand domain, although no endogenous ligand has yet been found. ErbB2 dimerizes with ErbB1, 3, and 4, and can activate different pathways including the PI3K, PLCγ, and MAPK pathways, depending on the ErbB receptor involved. Coupling with ErbB3 activates the PI3K pathway, whereas coupling with any of these three receptors can induce the MAPK pathway. ErbB2 signaling promotes cell proliferation and inhibits apoptosis. ErbB2 is overexpressed in various cancers and may play a role in the development and metastasis of gliomas, and ovarian, breast, lung, and gastric cancer.
Product Information
Format
Purified
Control
SK-BR-3 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-c-ErbB2/c-Neu Antibody, clone 3B5 is a Mouse Monoclonal Antibody for detection of c-ErbB2/c-Neu also known as MLN 19, Proto-oncogene Neu, Proto-oncogene c-ErbB-2 or CD340, has been validated in WB & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-ErbB2/c-Neu in human breast cancer tissue.
Biological Information
Immunogen
Linear peptide corresponding human c-ErbB2/c-Neu.
Clone
3B5
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001005862
Gene Symbol
ERBB2
HER2
MLN19
NEU
NGL
Purification Method
Protein G Purified
UniProt Number
P04626
Molecular Weight
~185 kDa observed. Uniprot describes four isoforms at ~138 kDa (isoform 1), ~63 kDa (isoform 2), ~71 kDa (isoform 3), and ~137 kDa (isoform 4), which can be highly glycosylated and phosphorylated. This antibody may detect one or more isoforms. Uncharacterized bands may be observed at ~27 kDa and ~38 kDa in some cell lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in SK-BR-3 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected c-ErbB2/c-Neu in 10 µg of SK-BR-3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
