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Millipore Use Anti-Mdm2 Antibody, Clone 4b2c1.11 (Mouse Monoclonal Antibody) Validated In Wb, Ip, Icc To Detect Mdm2 Also Known As E3 Ubiquitin-Protein Ligase Mdm2, Double Minute 2 - Mill (Additional S&H or Hazmat Fees May Apply)
MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Product Information
Format
Purified
Control
Mouse brain tissue lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Use Anti-MDM2 Antibody, clone 4B2C1.11 (mouse monoclonal antibody) validated in WB, IP, ICC to detect MDM2 also known as E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cell lysates (Hjerpe, R., et al. (2010). 42(5):725-735.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cells (Hjerpe, R., et al. (2010). 42(5):725-735.).
Biological Information
Immunogen
Recombinant protein corresponding to human MDM2.
Clone
4B2C1.11
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002383
Gene Symbol
MDM2
Purification Method
Protein G Purified
UniProt Number
Q00987
Molecular Weight
~55 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected MDM2 in 10 µg of mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
