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Millipore Use Anti-N-Myc Antibody, Clone Ncm Ii 100 (Mouse Monoclonal Antibody) Validated In Wb, Ip, Icc To Detect N-Myc Also Known As N-Myc Proto-Oncogene Protein, Class E Basic Helix-Loop-Helix - Mill (Additional S&H or Hazmat Fees May Apply)
The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
Product Information
Format
Purified
Control
IMR-32 cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Use Anti-N-Myc Antibody, clone NCM II 100 (mouse monoclonal antibody) validated in WB, IP, ICC to detect N-Myc also known as N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Application Notes
Immunofluorescence Analysis: A representative lot from an independent laboratory detected N-Myc in IMR5 cells (Ikegaki, N., et al. (1986). 83(16):5929-5933.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated N-Myc in IP (Brondyk, W. H., et al. (1991). 6(7):1269-1276.).
Biological Information
Immunogen
Recombinant protein corresponding to human N-Myc.
Clone
NCM II 100
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_005369
Gene Symbol
MYCN
BHLHE37
NMYC
Purification Method
Protein G Purified
UniProt Number
P04198
Molecular Weight
~55 kDa observed. Uniprot describes a molecular weight at ~50 kDa. Uniprot states that this protein may be phosphorylated.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in IMR-32 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected N-Myc in 10 µg of IMR-32 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
