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Millipore Anti-P53 (Wild Type) Antibody, Clone Pab1620 Is A Mouse Monoclonal Antibody For Detection Of P53 (Wild Type) Also Known As Cellular Tumor Antigen P53, Tumor Suppressor P53 - Mill (Additional S&H or Hazmat Fees May Apply)
Wild-type p53 is present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.
Product Information
Format
Purified
Control
Mouse brain tissue lysate
Presentation
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-p53 (wild type) Antibody, clone PAb1620 is a Mouse Monoclonal Antibody for detection of p53 (wild type) also known as Cellular tumor antigen p53, Tumor suppressor p53 & has been validated in WB & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected p53 in normal human brain tissue.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p53 in IP (Lu, X., et al. (1992). Cell. 70(1):153-161.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in dorsal skin samples from animal trunks (Kramata, P., et al. (2005). Cancer Res. 65(9):3577-3585.) and in certain human cancer tissues (Cordon-Cardo, C., et al, (1991). 51(23 Pt 1):6372-6380.).
Biological Information
Immunogen
Mouse VLM tumor cells.
Clone
Pab1620
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001120705
Gene Symbol
Tp53
P53
Trp53
Purification Method
Protein G Purified
UniProt Number
P02340
Molecular Weight
~53 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p53 in mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
