The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Mdm2 Antibody, Clone If2 Is A Mouse Monoclonal Antibody For Detection Of Mdm2 Also Known As E3 Ubiquitin-Protein Ligase Mdm2 Or Hdm2 & Has Been Validated In Wb & Ihc. - Mill (Additional S&H or Hazmat Fees May Apply)
MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes the transcriptional activity of p53 in two main ways. The N-terminal of MDM2 interacts and disables the transactivation domain of p53. In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences. In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome. MDM2 may produce these effects in cooperation with the Mdmx protein. Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.
Product Information
Format
Purified
Control
A431 cell lysate
Presentation
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-MDM2 Antibody, clone IF2 is a Mouse Monoclonal Antibody for detection of MDM2 also known as E3 ubiquitin-protein ligase Mdm2 or Hdm2 & has been validated in WB & IHC.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blot Analysis: A representative lot detected MDM2 in Raji, HepG2, and Molt-4 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MDM2 in human prostate cancer tissue.
Biological Information
Immunogen
Recombinant protein corresponding to human MDM2.
Clone
IF2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_002383
Gene Symbol
MDM2
Purification Method
Protein G Purified
UniProt Number
Q00987
Molecular Weight
~85 kDa and ~60 kDa observed. Uniprot describes many isoforms between 12-56 kDa, which can be highly phosphorylated and ubiquitinated.
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MDM2 in 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
