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Millipore Detect The Phospho-Rnapii (Thr4) Protein Using This Anti-Phospho-Rnapii (Thr4), Clone 1g7 Validated For Use In Wb, Ip, Icc & Chip. - Mill (Additional S&H or Hazmat Fees May Apply)
RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Product Information
Format
Purified
Control
Untreated and lambda phosphatase treated HeLa cell lysates.
Presentation
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect the phospho-RNAPII (Thr4) protein using this Anti-phospho-RNAPII (Thr4), clone 1G7 validated for use in wb, IP, ICC & ChIP.
Key Applications
Western Blotting
Immunoprecipitation
Immunocytochemistry
Chromatin Immunoprecipitation (ChIP)
Application Notes
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cell extracts (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cells (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Thr4) in stably transfected Raji cell lysates (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).
Biological Information
Immunogen
Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Thr4.
Epitope
Phosphorylated Thr4
Clone
1G7
Host
Rat
Specificity
This antibody recognizes RNAPII phosphorylated at Thr4.
Isotype
IgG2aκ
Species Reactivity
Human
Mouse
Yeast
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000928
Gene Symbol
POLR2A
POLR2
Purification Method
Protein G Purified
UniProt Number
P24928
Molecular Weight
~220 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in untreated and lambda phosphatase treated HeLa cell lysates.
Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-RNAPII (Thr4) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
