The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore This Anti-Ezh2 Antibody, Clone Ae25 Is Validated For Use In Western Blotting, Ihc For The Detection Of Ezh2. - Mill (Additional S&H or Hazmat Fees May Apply)
EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis, and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.
Product Information
Format
Purified
Control
T47D cell lysate
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Western Blot Analysis: A 1:500 dilution from a representative lot detected EZH2 in Jurkat and HeLa cell lysates.
Immunohistochemistry Analysis: A representative lot detected EZH2 in breast carcinoma tissue (Pietersen et al., 2008)
Biological Information
Immunogen
MBP-tagged recombinant protein corresponding to human EZH2.
Western Blot Analysis: A 1:1000 dilution of this antibody detected EZH2 in 10 µg of T47D cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
